TB-500 (Thymosin Beta-4)
TB-500 is a synthetic form of Thymosin Beta-4 known for its powerful role in tissue regeneration, cell migration, and wound healing. It is widely researched for soft tissue repair, inflammation reduction, and promoting angiogenesis (new blood vessel formation).
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TB-500 is a synthetic version of a naturally occurring peptide known as Thymosin Beta-4. It is recognized for its role in tissue regeneration, cell migration, and wound healing. TB-500 is widely studied in experimental models of soft tissue repair, inflammation reduction, and cardiovascular healing. Its ability to promote cellular remodeling and increase angiogenesis makes it a valuable research compound for regeneration-focused applications.
โ Form: Lyophilized peptide
โ Purity: >99%
โ Origin: Manufactured and tested in the USA
โ Certifications: Certificate of Analysis (COA) included with every order
TB-500 mimics the effects of Thymosin Beta-4 by:
โ Enhancing cell migration and differentiation
โ Promoting angiogenesis (formation of new blood vessels)
โ Reducing inflammatory responses at injury sites
โ Supporting actin binding and cytoskeletal remodeling
โ Wound healing and soft tissue regeneration
โ Muscle and ligament recovery studies
โ Inflammation modulation and scar reduction
โ Cardiovascular tissue healing models
โ Ophthalmic and corneal repair studies
โ Versatile applications across organ systems
โ Rapid onset of action in tissue injury models
โ Synergistic potential with other regenerative peptides
โ Favorable stability and low immunogenicity
Each vial is:
โ Produced in a GMP-compliant U.S. facility
โ Supplied with a Certificate of Analysis (COA)
โ Tested via HPLC and mass spectrometry
โ Shipped securely from the USA
โ Contents: 5mg or 10mg of lyophilized TB-500 peptide
โ Appearance: White/off-white powder
โ Solubility: Sterile bacteriostatic water recommended
โ Storage: Store lyophilized powder at -20ยฐC; stable for up to 30 days post-reconstitution if refrigerated
*For laboratory research use only. Not intended for human or veterinary use.*
โ Sold strictly for research purposes
โ Not for human consumption or diagnostic use
โ Researchers must adhere to applicable legal and institutional safety protocols
TB-500 is the synthetic version of a naturally occurring peptide known as Thymosin Beta-4. It is widely researched for its role in cellular regeneration, tissue repair, angiogenesis, and inflammation modulation.
TB-500 promotes cell migration and angiogenesis by upregulating actin-binding proteins. It plays a crucial role in tissue healing by supporting new blood vessel formation, reducing inflammation, and improving flexibility at the site of injury in research models.
Shop Peptides Direct supplies TB-500 as a lyophilized powder in sterile, research-grade vials. Our compounds are made in the USA and undergo rigorous lab testing to ensure consistency, sterility, and purity.
Store the lyophilized vial at -20ยฐC in a dry, light-protected environment. Once reconstituted, keep the solution refrigerated at 2โ8ยฐC and use within your labโs recommended timeframe to maintain integrity.
Each vial of TB-500 is โฅ98% pure, confirmed through HPLC and Mass Spectrometry. A batch-specific Certificate of Analysis (COA) is included with every purchase, so your lab has complete transparency on quality.
Yes. While TB-500 is stable during shipping in our standard packaging, cold-chain shipping is available on request for sensitive applications or bulk research orders.
In preclinical settings, TB-500 is sometimes studied alongside other healing peptides such as BPC-157 or growth factors. All use should comply with approved lab protocols and sterile handling procedures.
Visit our Lab Test Results page, choose TB-500 from the dropdown menu, and enter your email. Weโll send you the full COA for the batch youโve ordered.
We guarantee โฅ98% purity, verified lab testing, and U.S.-based manufacturing. Every order includes a COA, and our Price Match Guarantee ensures you receive the best value for your research budgetโif you find comparable quality for less, weโll match it.
TB-500 (5 mg & 10 mg Vial) Dosage Protocol
Quickstart Highlights
TBโ500 is a synthetic peptide fragment corresponding to the active region of thymosin betaโ4 (Tฮฒ4), a naturally occurring 43โaminoโacid protein involved in tissue repair and regeneration[1][2]. This educational protocol presents a onceโdaily subcutaneous approach using practical dilutions for accurate insulinโsyringe measurements in research settings.
5 mg Vial
- Reconstitute: Add 3.0 mL bacteriostatic water โ ~1.67 mg/mL concentration.
- Typical daily range: 500โ1000 mcg once daily (gradual titration recommended).
- Easy measuring: At 1.67 mg/mL, 1 unit = 0.01 mL โ 16.7 mcg on a Uโ100 insulin syringe.
10 mg Vial
- Reconstitute: Add 3.0 mL bacteriostatic water โ ~3.33 mg/mL concentration.
- Typical daily range: 500โ1000 mcg once daily (gradual titration recommended).
- Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL โ 33.3 mcg on a Uโ100 insulin syringe.
Storage: Lyophilized: store at โ20 ยฐC (โ4 ยฐF); after reconstitution, refrigerate at 2โ8 ยฐC (35.6โ46.4 ยฐF); do not freeze reconstituted solution.
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Standard / Gradual Approach
5 mg Vial (3 mL = ~1.67 mg/mL)
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1โ2 | 500 mcg | 30 units (0.30 mL) |
| Weeks 3โ4 | 600 mcg | 36 units (0.36 mL) |
| Weeks 5โ8 | 750 mcg | 45 units (0.45 mL) |
| Weeks 9โ12 | 1000 mcg | 60 units (0.60 mL) |
10 mg Vial (3 mL = ~3.33 mg/mL)
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1โ2 | 500 mcg | 15 units (0.15 mL) |
| Weeks 3โ4 | 600 mcg | 18 units (0.18 mL) |
| Weeks 5โ8 | 750 mcg | 23 units (0.23 mL) |
| Weeks 9โ12 | 1000 mcg | 30 units (0.30 mL) |
Frequency: Inject once daily subcutaneously. These schedules use the largest practical dilution (3.0 mL) to keep perโinjection units in a comfortable range for accurate measurement. Total weekly dose averages ~5 mg, consistent with research protocols[3][4].
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label with date and concentration; refrigerate at 2โ8 ยฐC (35.6โ46.4 ยฐF), protected from light.
Supplies Needed
Plan based on an 8โ16 week daily protocol with gradual titration.
5 mg Vials (TBโ500, 5 mg each)
- 8 weeks โ 8 vials
- 12 weeks โ 12 vials
- 16 weeks โ 16 vials
10 mg Vials (TBโ500, 10 mg each)
- 8 weeks โ 4 vials
- 12 weeks โ 7 vials
- 16 weeks โ 10 vials
Insulin Syringes (Uโ100)
- Per week: 7 syringes (1/day)
- 8 weeks: 56 syringes
- 12 weeks: 84 syringes
- 16 weeks: 112 syringes
Bacteriostatic Water (10 mL bottles)
Use ~3.0 mL per vial for reconstitution.
- 5 mg protocol (8 weeks, 8 vials): 24 mL โ 3 ร 10 mL bottles
- 10 mg protocol (8 weeks, 4 vials): 12 mL โ 2 ร 10 mL bottles
- 10 mg protocol (12 weeks, 7 vials): 21 mL โ 3 ร 10 mL bottles
Alcohol Swabs
One for the vial stopper + one for the injection site each day.
- Per week: 14 swabs (2/day)
- 8 weeks: 112 swabs โ recommend 2 ร 100โcount boxes
- 12 weeks: 168 swabs โ recommend 2 ร 100โcount boxes
- 16 weeks: 224 swabs โ recommend 3 ร 100โcount boxes
Protocol Overview
Concise summary of the onceโdaily regimen.
- Goal: Support tissue repair, wound healing, and angiogenesis through the active thymosin betaโ4 fragment mechanism[5][6].
- Schedule: Daily subcutaneous injections for 8โ12 weeks (extend to 16 weeks if research goals require).
- Dose Range: 500โ1000 mcg daily with gradual titration (~5 mg/week average).
- Reconstitution: 3.0 mL per vial (1.67 mg/mL for 5mg, 3.33 mg/mL for 10mg) for accurate unit measurements.
- Storage: Lyophilized frozen; reconstituted refrigerated; avoid freezeโthaw cycles of reconstituted solution.
Dosing Protocol
Suggested daily titration approach.
- Start: 500 mcg daily; increase by ~100โ150 mcg every 2 weeks as tolerated.
- Target: 750โ1000 mcg daily by Weeks 5โ12.
- Frequency: Once per day (subcutaneous).
- Cycle Length: 8โ12 weeks; optional extension to 16 weeks based on research protocol.
- Timing: Any consistent time daily; rotate injection sites systematically.
Storage Instructions
Proper storage preserves peptide quality and activity.
- Lyophilized: Store at โ20 ยฐC (โ4 ยฐF) in dry, dark conditions; minimize moisture exposure[7].
- Reconstituted: Refrigerate at 2โ8 ยฐC (35.6โ46.4 ยฐF); do not freeze reconstituted solution as freezing can denature peptides.
- Allow vials to reach room temperature before opening to reduce condensation uptake.
- Use reconstituted vials within 28 days when stored with bacteriostatic water preservative[8].
Important Notes
Practical considerations for consistency and safety in research protocols.
- Use new sterile insulin syringes for each injection; dispose in a sharps container.
- Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation and lipohypertrophy[9].
- Inject slowly; wait a few seconds before withdrawing the needle to prevent backflow.
- Document daily dose, injection site, and any observations to maintain consistency.
- Regulatory Note: TBโ500 is banned by WADA for athletic use and is not FDAโapproved for human administration[10].
How This Works
TBโ500 represents the Nโterminal active fragment of thymosin betaโ4, specifically the heptapeptide sequence AcโLKKTETQ[1][2]. This region is responsible for the actinโbinding and cellโmigration properties of the full thymosin molecule. Preclinical studies demonstrate that TBโ500 promotes angiogenesis, accelerates wound healing, and supports tissue regeneration by upregulating cell motility and blood vessel formation[5][6]. Research in animal models shows enhanced collagen deposition and reduced healing time in injury sites treated with thymosin fragments[11]. Recent metabolic studies suggest TBโ500 may act as a prodrug, cleaving to an active pentapeptide metabolite that drives biological activity[12].
Potential Benefits & Side Effects
Observations from preclinical and veterinary literature.
- Supports accelerated wound healing and tissue repair through enhanced angiogenesis and cell migration[5][6].
- May reduce inflammation and fibrosis indirectly via thymosin pathways observed in animal models[11].
- Generally well tolerated in veterinary studies; occasional mild injectionโsite reactions (redness, tenderness) reported.
- Human safety data is limited; no largeโscale clinical trials have been completed for TBโ500 specifically[13].
Lifestyle Factors
Complementary strategies for optimal research outcomes.
- Maintain adequate protein intake to support tissue repair and regeneration processes.
- Combine with appropriate physical activity; avoid overtraining during injury recovery phases.
- Prioritize sleep (7โ9 hours) to maximize natural recovery and repair mechanisms.
- Manage stress levels through evidenceโbased practices to support overall healing.
Injection Technique
General subcutaneous guidance from clinical bestโpractice resources[14][15].
- Clean the vial stopper and skin with alcohol; allow to air dry completely.
- Pinch a skinfold at the injection site; insert the needle at 45โ90ยฐ into subcutaneous tissue[14].
- Do not aspirate for subcutaneous injections; inject slowly and steadily[14].
- Rotate sites systematically within approved areas (abdomen, thighs, upper arms) to avoid lipohypertrophy[9].
- Wait 5โ10 seconds after injection before withdrawing needle to prevent medication leakage.
Recommended Source
We recommend Shop Peptides Direct for highโpurity TBโ500 (5 mg & 10 mg).
Why Shop Peptides Direct?
- Highโpurity peptides (โฅ99%) with thirdโparty testing and batchโspecific COAs.
- Consistent quality control and ISOโaligned handling procedures.
- Reliable fulfillment with proper coldโchain shipping to maintain peptide integrity.
- Transparent documentation for research compliance.
Important Note
This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment. TBโ500 is not approved by the FDA for human use and is for research purposes only. TBโ500 is a prohibited substance in competitive sports under WADA regulations.
References
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FASEB Journal โ Biological activities of thymosin ฮฒ4 defined by active peptide sequences (TBโ500 fragment identification)
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Journal of Chromatography A (PubMed) โ Doping control analysis of TBโ500 as synthetic thymosin ฮฒ4 fragment in biological samples
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WADA Scientific Research โ Investigation of TBโ500 metabolism, synthesis of metabolites, and detection limits
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Racing Medication & Testing Consortium โ Thymosin ฮฒ4 regulatory bulletin (TBโ500 use in equine sports medicine)
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Journal of Investigative Dermatology (PubMed) โ Thymosin beta4 accelerates wound healing (preclinical wound healing model)
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FASEB Journal (PubMed) โ Active site mapping of thymosin ฮฒ4 fragments for angiogenesis and cell migration
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Verified Peptides Storage Guide โ Lyophilized peptide storage best practices (temperature, humidity, light protection)
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Empower Pharmacy โ Bacteriostatic water injection guidelines (0.9% benzyl alcohol, multiโdose vial stability)
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NCBI Bookshelf โ Best practices for subcutaneous injection (aseptic technique, site rotation)
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WADA Prohibited List โ TBโ500 classification as prohibited substance in competitive sports
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Journal of Investigative Dermatology โ Thymosin ฮฒ4 wound healing mechanisms (collagen deposition, angiogenesis, granulation tissue)
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Journal of Chromatography B (PubMed) โ Quantification of TBโ500 metabolites and wound healing activity screening (prodrug hypothesis)
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